Williams' Medium E Description Williams' Medium E（WE）was formulated to support long-term cell cultures of adult rat liver epithelial cells, originally developed by Williams and Gunn as reduced serum-supplemented medium. This WE can also be used for growing hepatocyte cells of different species This WE is a modification of earlier media that has been enriched in amino acids and double the glucose. Otherwise contains unique ingredients, including zinc, iron, manganese, non-essential amino acids, the reducing agent glutathione and the lipid methyl linoleate. It contains no proteins or growth factors. Therefore, it requires supplementation, commonly with 5-10% Fetal Bovine Serum (FBS). WE uses a sodium bicarbonate buffer system (2.2 g/L) and therefore requires a 5-10% CO2 environment to maintain physiological PH. This WE is modified with Phenol Red, NaCO3, Sodium Pyruvate and HEPES, without L-glutamine. Product Williams' Medium E Catalog No. LV-WME001 Form Liquid Volume 100/500ml Storage and Shipping Condition 2-8°C. Protect from light Information For Research Use Only. Not intended for animal or human diagnostic or therapeutic use. Technical support For any questions related to this protocol, contact us at: Tel: 0755-28284050，Mob: 19902901483(Dr.Zhou)